chromatography & spectroscopy
276
THINLAYERCHROMATOGRAPHY
SILG -
“unmodified standard silica layers”
ALUGRAM -
“Xtra” - “SILG”
Thin Layer Chromatography (TLC), like all chromatographic
techniques, based on multi-stage distribution process
involving;
Although the TLC principle is known more than 100 years,
today TLC has gained increasing importance.
Applicability of TLC was enhanced by development of new
absorbants and supports.
The basic steps of TLC are the following;
Sample preparation. Sample application. Development
of chromatogram. Evaluation in TLC
Silica 60, specific surface (BET) - 500 m2/g, mean pore
size 60A, specific pore volume 0,75 ml/g, particle size 5 - 17
µm:standard grade.
Thickness of layer for analytical plates 0,25 mm. For
preparative plates 0,5 and 1,0 mm. For 2,0 mm preparative
layers a slightly closer material is used.
Indicators: manganese activated zinc silicate with green
fluorescence for short-wave UV ( 254 nm ); special inorganic
fluorescent pigment with blue fluorescence for long-wave UV
(366 nm).
Binders: highly polimeric products, which are stable in
almost all organic solvents and resistant towards aggressive
visualisation reagents.
Silica 60, specific surface (BET) - 500 m2/g, mean pore size
60A, specific pore volume 0,75 ml/g, particle size 5 - 17 µm
standard grade.
Binders: highly polimeric products, which are stable in
almost all organic solvents and resistant towards aggressive
visualisation reagents, also completely stable in purely
aqueous eluents.
Suitable absorbants ( as stationary phase ) coated
as a thin layer onto a suitable support such as glass,
aluminium or polyester sheet.
Solvents or solvent mixtures as the mobile phase.
Sample molecules
glass sheet
polyester sheet
aluminum sheet
Offers outstanding advatages as;
Outstanding wettebility for precise colorization results,
even with 100 % aqueous detection reagents.
Best separation efficiency and reproducibility for each lot.
Easy and reliable cutting due to an optimized binder
system without flaking of silica.
dimensions
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
20 x 20 cm
thickness
of layer
0,25 mm
0,25 mm
0,25 mm
0,50 mm
0,50 mm
1,00 mm
1,00 mm
2,00 mm
2,00 mm
0,20 mm
0,20 mm
0,20 mm
0,20 mm
0,20 mm
0,20 mm
material
glass sheet
glass sheet
glass sheet
glass sheet
glass sheet
glass sheet
glass sheet
glass sheet
glass sheet
polyester sheet
polyester sheet
aluminium sheet
aluminium sheet
aluminium sheet
aluminium sheet
pack
quantity
25 pieces
25 pieces
25 pieces
20 pieces
20 pieces
15 pieces
15 pieces
12 pieces
12 pieces
25 pieces
25 pieces
25 pieces
25 pieces
25 pieces
25 pieces
108.01.001
108.01.002
108.01.003
108.01.004
108.01.005
108.01.006
108.01.007
108.01.008
108.01.009
108.01.010
108.01.011
108.01.012
108.01.013
108.01.014
108.01.015
catalogue
number
product
descriptions
TLC precoated plates SILG-25
TLC precoated plates SILG-25 / UV 254
TLC precoated plates SILG-25 / UV 254 + 366
TLC precoated plates SILG-50
TLC precoated plates SILG-50 / UV 254
TLC precoated plates SILG-100
TLC precoated plates SILG-100 / UV 254
TLC precoated plates SILG-200
TLC precoated plates SILG-200 / UV 254
POLYGRAM sheets SILG
POLYGRAM sheets SILG / UV 254
ALUGRAM sheets SILG
ALUGRAM sheets SILG / UV 254
ALUGRAM “Xtra” sheets SILG
ALUGRAM” Xtra”sheets SILG / UV 254