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chromatography & spectroscopy

276

THINLAYERCHROMATOGRAPHY

SILG -

“unmodified standard silica layers”

ALUGRAM -

“Xtra” - “SILG”

Thin Layer Chromatography (TLC), like all chromatographic

techniques, based on multi-stage distribution process

involving;

Although the TLC principle is known more than 100 years,

today TLC has gained increasing importance.

Applicability of TLC was enhanced by development of new

absorbants and supports.

The basic steps of TLC are the following;

Sample preparation. Sample application. Development

of chromatogram. Evaluation in TLC

Silica 60, specific surface (BET) - 500 m2/g, mean pore

size 60A, specific pore volume 0,75 ml/g, particle size 5 - 17

µm:standard grade.

Thickness of layer for analytical plates 0,25 mm. For

preparative plates 0,5 and 1,0 mm. For 2,0 mm preparative

layers a slightly closer material is used.

Indicators: manganese activated zinc silicate with green

fluorescence for short-wave UV ( 254 nm ); special inorganic

fluorescent pigment with blue fluorescence for long-wave UV

(366 nm).

Binders: highly polimeric products, which are stable in

almost all organic solvents and resistant towards aggressive

visualisation reagents.

Silica 60, specific surface (BET) - 500 m2/g, mean pore size

60A, specific pore volume 0,75 ml/g, particle size 5 - 17 µm

standard grade.

Binders: highly polimeric products, which are stable in

almost all organic solvents and resistant towards aggressive

visualisation reagents, also completely stable in purely

aqueous eluents.

Suitable absorbants ( as stationary phase ) coated

as a thin layer onto a suitable support such as glass,

aluminium or polyester sheet.

Solvents or solvent mixtures as the mobile phase.

Sample molecules

glass sheet

polyester sheet

aluminum sheet

Offers outstanding advatages as;

Outstanding wettebility for precise colorization results,

even with 100 % aqueous detection reagents.

Best separation efficiency and reproducibility for each lot.

Easy and reliable cutting due to an optimized binder

system without flaking of silica.

dimensions

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

20 x 20 cm

thickness

of layer

0,25 mm

0,25 mm

0,25 mm

0,50 mm

0,50 mm

1,00 mm

1,00 mm

2,00 mm

2,00 mm

0,20 mm

0,20 mm

0,20 mm

0,20 mm

0,20 mm

0,20 mm

material

glass sheet

glass sheet

glass sheet

glass sheet

glass sheet

glass sheet

glass sheet

glass sheet

glass sheet

polyester sheet

polyester sheet

aluminium sheet

aluminium sheet

aluminium sheet

aluminium sheet

pack

quantity

25 pieces

25 pieces

25 pieces

20 pieces

20 pieces

15 pieces

15 pieces

12 pieces

12 pieces

25 pieces

25 pieces

25 pieces

25 pieces

25 pieces

25 pieces

108.01.001

108.01.002

108.01.003

108.01.004

108.01.005

108.01.006

108.01.007

108.01.008

108.01.009

108.01.010

108.01.011

108.01.012

108.01.013

108.01.014

108.01.015

catalogue

number

product

descriptions

TLC precoated plates SILG-25

TLC precoated plates SILG-25 / UV 254

TLC precoated plates SILG-25 / UV 254 + 366

TLC precoated plates SILG-50

TLC precoated plates SILG-50 / UV 254

TLC precoated plates SILG-100

TLC precoated plates SILG-100 / UV 254

TLC precoated plates SILG-200

TLC precoated plates SILG-200 / UV 254

POLYGRAM sheets SILG

POLYGRAM sheets SILG / UV 254

ALUGRAM sheets SILG

ALUGRAM sheets SILG / UV 254

ALUGRAM “Xtra” sheets SILG

ALUGRAM” Xtra”sheets SILG / UV 254